1.Is it possible to wash out application of TLC-S (200 M) in order to apply bile acid to the same cell several times (reproducibility of responses)?
2.If the answer is “YES”, does the second application of the same dose of TLC-S (200M) have the same effect on intracellular calcium concentration? Areas under the curve of experimental traces with TLC-S-induced cytosolic Ca2+ elevation induced by first and second additions of 200 M TLC-S should be compared and evaluated.