Discuss Is it possible to wash out application of TLC-S (200 M) in order to apply bile acid to the same cell several times (reproducibility of responses)?

1.Is it possible to wash out application of TLC-S (200 M) in order to apply bile acid to the same cell several times (reproducibility of responses)?

2.If the answer is “YES”, does the second application of the same dose of TLC-S (200M) have the same effect on intracellular calcium concentration? Areas under the curve of experimental traces with TLC-S-induced cytosolic Ca2+ elevation induced by first and second additions of 200 M TLC-S should be compared and evaluated.