Chloroplast
What would happen to the chloroplasts if you resuspended them in water instead of in suspension buffer?
Would the amount of chlorophyll be affected by resuspending the chloroplasts in water? If so, would the measured amount increase or decrease?
Which type of tissue contains more chlorophyll per gram of tissue (you can tell this just by looking at which leaf is darker green)?
We do not know (and cannot determine from this experiment) how many chloroplasts are in an individual cell in either tissue. What are the two possible explanations for why one tissue has more chlorophyll than the other (hint- think about how many chloroplasts are present in each possibility)?
What was the concentration of chloroplasts in both of your suspensions?
What was the concentration of chlorophyll in both of your suspensions?
What was the average amount of chlorophyll per chloroplast in each of your suspensions? (also write these values on the board)
Using your results only, why do you think one of your samples was darker green than the other (choose one of the answers from question 4) ?
Using the results of the entire class perform a t-test to see if the amount of chlorophyll per chloroplast is different between the two tissue types. Report your answer in an appropriate format.
Which answer to question 4 is supported by this t-test?
How much sorbitol (182 g/mol) do you need to make 25 ml of grinding buffer?
EDTA (372 g/mol) comes as a 0.5 M stock solution. What volume do you need to make 5 ml of suspension buffer?