Describe the virion structure of both viruses:
Capsid symmetry
Type of nucleic acid (number of strands, + vs. -)
Presence or absence of an envelope (membrane)
Discuss how each virus binds to and enters the cell (including receptors, if known)
Discuss how and where the single-stranded DNA molecules in the original virus capsids are converted to double-stranded DNA molecules, and by which enzymes in each case (host or viral).
Discuss where transcription happens for both viruses (and which enzymes..host or viral…do the transcribing)
For Parvoviruses, what process causes a single primary transcript to produce a variety of different mRNAs that can then be translated into the various viral proteins?
For Parvoviruses, where and by what process are progeny ssDNAs made? Where does the DNA polymerase that is used come from? Where does the helicase come from?
For Hepatitis B virus, discuss how the use of different promoters, alternative reading frames and different start codons allows it to make 4 mRNAs and 7 proteins.
For Hepatitis B virus, outline the process by which the pre-genome RNA is replicated into partially double-stranded DNA. You do not need to go into all of the details regarding the direct repeats, but you will need to discuss which enzyme does the work, where the replication takes place, and the order in which DNA strands are synthesized (steps 8, 9 and 10 in the overview figure).
The various types of disease symptoms and modes of transmission for both viruses.
How the HBV vaccine works.
Define and/or discuss the function of
Inverted repeats in the parvovirus genome
HBV S, M, L, HBeAg, HBcAg and P proteins
Rolling Hairpin replication in parvoviruses (you don’t need to know how it works, just what it is and how it is used).