Chapter 14 Learning Objectives
DNA: The Genetic Material
Understand Griffith’s experiment and how it showed transformation of bacteria occurred in the mice (Figure 14.1).
Understand how scientists showed that DNA was the “transforming principle”.
Understand the Hershey-Chase Experiment (Viral transformation confirmed DNA was the hereditary information, not protein)
Section 14.2
Know the detailed molecular structure of DNA? (Figure 14.3)
What is the overall 3-D structure of DNA? (Watson and Crick DNA molecule)
What are the purines and pyrimidines of nucleic acids?
What are the base-pairing rules for RNA and DNA?
Section 14.3
What does it mean that DNA replication is semi-conservative?
Section 14.4
How do prokaryotes replicate? (Figure 14.13)
What do: DNA polymerase I, II and III do in Coli?
Explain the following enzymes and their job in DNA replication:- Helicase polymerase, topisomerase, DNA gyrase, DNA ligase, DNA primase
Explain how the leading and lagging strands of DNA replication are produced differently.
How is prokaryotic DNA replication different than eukaryotic DNA replication? (also part of Section 14.5)