Given below is Figure 3(A) which was presented in the Journal of Neuroinflammation (2007).
Figure 3A: Representative photomicrographs showing RT-PCR products of COX-1, COX-2, mPGES-1, and β-actin mRNAs.
Your task is specific to the figure above. In your essay, you are required to
⦁ Discuss the main technique used, including the principle of the technique, key steps, advantages and disadvantages and use of controls.
⦁ Describe in what context the authors have used this method.
⦁ Provide a concise background including the biology that underpins what is being explored in this figure.
⦁ Explain systematically the results of this figure including the different treatment conditions and/or time points, genes analysed and the results.
⦁ Interpret and discuss key findings. Are the results expected or were there any unexpected results? Was the method appropriate for this investigation? Were appropriate controls used?
⦁ Provide key conclusions and future steps based on the figure interpretation and discussion