Use chromatography to purify a protein of interest. Use a colorimetric assays to examine protein activity and folding. Determine the inhibition of several antibiotics with the protein. Determine how various reagents alter the stability/activity of the protein. Examine protein purity using gel electrophoresis. Generate a protein concentration standard curve and calculate the concentration of your […]
Tag Archives: chromatography
Identify an unknown analgesic using Thin-Layer Chromatography (TLC).
Reparaphrase the lab report Objectives Given samples of acetylsalicylic acid, acetaminophen, caffeine, and ibuprofen, calculate the Rf values and melting temperatures. Identify solvent system that produces good separation. Identify an unknown analgesic using Thin-Layer Chromatography (TLC). Principle “Thin-layer chromatography is a method of separation, or identification of a mixture of components by using finely divided […]
What is GAL80 and why does the yeast two hybrid system require mutant strains of yeast that lack the expression of this protein?
The authors state that “protein-protein interactions between two proteins have generally been studied using biochemical techniques such as cross-linking, co-immunoprecipitation and co-fractionation by chromatography.” Briefly describe one of these techniques. What does the GAL4 protein normally do in Saccharomyces cerevisiae (Brewer’s yeast)? What are the attributes of GAL4 that enable it to be used in […]
