How do various reagents, such as urea, hydrogen peroxide and change in pH, affect protein activity and stability?

Use chromatography to purify a protein of interest. Use a colorimetric assays to examine protein activity and folding. Determine the inhibition of several antibiotics with the protein. Determine how various reagents alter the stability/activity of the protein. Examine protein purity using gel electrophoresis. Generate a protein concentration standard curve and calculate the concentration of your […]

Explain what techniques might be used in genetic engineering and what types of organisms are genetically engineered and why they have been created.

Chapter 17 Learning Objectives Biotechnology Definitions of restriction enzymes, recombinant DNA Explain how DNA gel electrophoresis works, DNA fingerprinting What are different uses of biotechnology? Give three examples of genetically engineered organisms and why they were created. Explain what techniques might be used in genetic engineering and what types of organisms are genetically engineered and […]